Sequencing by Ligation with Double-Labeled Fluorescent Probes
نویسندگان
چکیده
We have synthesized a set of double-labeled fluorescent probes and evaluated the performance of these fluorescent probes in sequencing by ligation (SBL). The results indicated that the ligation efficiency and specificity of double-labeled fluorescent probes was as higher as that of singe-labeled fluorescent probes. Moreover, double-labeled f luorescent p robes y ielded a r emarkable i ncrease i n s ignal i ntensities co mpared t o s ingle-labeled f luorescent probes. I t could also reduce background and shorten exposition time compared to probes labeled with single FAM and FITC dyes. Thus, it could supplant singe-labeled fluorescent probes in SBL to increase sequencing accuracy.
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